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| JULY
2009 |
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Bio-Rad Newsletter |
Not enough protein? 10mg is all you need.
Check out the latest addition in our Proteominer
Family |
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ProteoMiner technology is a novel
sample enrichment tool for reducing the dynamic range of
protein concentration in complex biological samples
(serum, plasma and other sample types).
Multiple kits are available to meet the needs of
downstream applications:
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Key Benefits of
ProteoMiner
- Eliminates the dependency for a predefined
set of antibodies
- Enriches and concentrates low-abundance
proteins that cannot be detected through
traditional methods such as immunodepletion
- Convenient spin column format for easy of
use
- Compatible with all major downstream protein
analysis techniques
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Click to read
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Click to
read | | |
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Protein Separation and Analysis
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Bio-Rad offers a wide range of products from sample
preparation to profiling and quantitation. Read on to
see the respective product offerings.
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Simplify
protein sample preparation with Bio-Rad’s comprehensive array
of products.
For more information on Sample preparation, please click
the following links.
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PROTEIN SEPARATION |
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2-D
electrophoresis is used to separate complex protein
samples based on pI and molecular weight. IEF is primarily
used as the first dimension in 2-D analysis.
| 2-D electrophoresis |
Liquid-Phase IEF |
The Protean IEF system, includes
Protean IEF cell and ReadyStrip IPG, is optimized to
perform first dimension IEF. This system offers a number
of advantages:
- streamlined handling,
- enhanced resolution,
- increased reproducibility
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The MicroRotofor
cell is a preparative liquid-phase isoelectric focusing
(IEF) device which fractionates microscale (~2.5 ml)
protein samples into 10 fractions according to
isoelectric point (pI) of protein samples in solution.
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Key Benefits
- High-capacity running platform holds 1 large or 2
mini trays, allowing up to twenty-four 7cm strips per
run or twelve 11, 17, 18 or 24-cm strips
- Integrated 10,000 V power supply that helps to
achieve desired volt-hours faster
- Optional thermal printer to provide a permanent
record of the programmed method and run data
- Preset methods and real-time editing for maximum
flexibility
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Key Benefits
- Accommodates small volume of samples (2.5ml)
- Separates samples into ten fractions
- Samples can be run in native or denaturing
conditions
- Offers two cooling settings for maintaining
protein integrity (10 and 20°C)
- Need not concentrate crude protein extracts before
MicroRotofor cell
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Profiling and
Quantitation |
| Molecular Imager FX?Pro and FX
Pro Plus System |
VersaDoc?Model 4000
System |
GS-800?
Densitometer |
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- High-specificity detection of multicolor
fluorescence
- Patented fiber-optic scanning system that provides
quick scan times with high sensitivity and resolution
- Wide Dynamic range
- Direct epi-scanning for increased collection
efficiency and reduction of
autofluorescence
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- High-resolution gel imaging
- Single- and multicolor fluorescent,
chemiluminescent, chemifluorescent, and colorimetric
samples
- Cooled CCD technology for great sensitivity
- Excellent data uniformity using flat
fielding
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- Calibrated transmissive and reflective scanning
- 12-bit data and 36.3um resolution, allowing
analysis of closest spots on a gel
- Accurate quantitation of stained gels within a
large dynamic range(0-3.0 OD)
- Unique design to accomodate wet samples of
variable thickness
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PD Quest software controls the
imaging systems, collects and manages large amounts of
image data, and rapidly and reliably analyzes the
information.
It facilitates the entire analysis
workflow from image to identification.
PDQuest combines:-
1) Automated spot matching
algorithms
2) Normalization between gel images
3)
Statistical and qualitative query reports
4) Merging
of multiple images of the same gel
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REFERENCES AND
PUBLICATIONS |
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Read more on the ProteoMiner technology in the recent
published paper by Boschetti E., Righetti P.G.
The ProteoMiner in the proteomic arena: a non-depleting
tool for discovering low-abundance species. J. of
Proteomics, 71, (2008), 255-264.
In-depth exploration of cows whey
proteome via combinatorial peptide ligand libraries. J.
of Proteomics 2 July 2009
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TIPS AND TECHNIQUES |
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Techniques such as 2-D electrophoresis (2DE) have been
used for nearly 30 years to separate proteins. Like any other
protein separation method, 2DE does have certain limitations
in dealing with high molecular weight and membrane proteins.
Bio-Rad has been in the electrophoresis business for years and
helped customers answered many challenging questions related
to 2DE analysis.
For resources and tips on proteomics, please visit
Bio-Rad’s the 2-D Doctor, at http://campaigner.e-dm2.com/redirect/13txunlqc4zkw4cos4kc00kc000o48w0/1A/1q2mo07pvj1cg0k80kccg084g8s8cokw/F/
Bio-Rad has recently participated in the quest for
reproducibility study in challenges of techniques used for
proteomics called “Fixing Proteomics? Details of the study
and experimental protocols for proteomics can be found in http://campaigner.e-dm2.com/redirect/64rdovdfs2w4scksswk880s48gkc4cgo/1A/1q2mo07pvj1cg0k80kccg084g8s8cokw/F/.
For the complete article on ProteoMiner Protein Enrichment
kits (Pg 29), click here.
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