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Muse® EGFR-RTK Activation Dual Detection Kit

Muse® EGFR-RTK Activation Dual Detection Kit

MCH200102


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Description

The Muse EGFR-RTK Activation Dual Detection Kit includes two directly conjugted antibodies—a phospho-specific anti-phospho-EGFR (Tyr1173)-Alexa Fluor® 555 and an anti-EGFR-PECy5 conjugated antibody—to measure total levels of EGFR expression. This two-color kit is designed to detect EGFR pathway activation by measuring EGFR phosphorylation relative to the total EGFR expression in any given cell population. By doing this, the levels of both the total and phosphorylated protein can be measured simultaneously in the same cell, resulting in a normalized and accurate measurement of EGFR activation after stimulation. Moreover, simultaneous measurement of both total and phospho-EGFR confirms target specificity of the phosphorylation event. Data generated using the Muse System provides statistical values, measuring the Percentage of inactivated cells / activated cells (via phosphorylation) / non-expressing cells

Additional details
  • pathway
  • protocol
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  • 物流專送

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  • 匯款
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