Muse® PI3K Activation Dual Detection Assay Kit

The Muse PI3K Activation Dual Detection Kit includes two directly conjugated antibodies—a phospho-specific anti-phospho-Akt (Ser473), Alexa Fluor® 555 and an anti-Akt, PECy5 conjugated antibody—to measure total levels of Akt. This two-color kit is designed to measure Akt phosphorylation relative to the total Akt expression in any given cell population. By doing this, the levels of both the total and phosphorylated protein can be measured simultaneously in the same cell, resulting in a normalized and accurate measurement of PI3K activation after stimulation. Moreover, simultaneous measurement of both total and phospho-Akt confirms target specificity of the phosphorylation event. Together, a total and phospho antibody duo performed in multiplex provides enhanced and more reliable detection of the phospho:total ratio within a mixed population. Muse System provides statistical values, measuring the Percentage of inactivated cells / activated cells (via phosphorylation) / non-expressing cells